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Butyrate and propionate are HDAC inhibitors that epigenetically regulate gene expression

Recently, both the FDA and the United States National Heart, Lung and Blood Institutes (NHLBI) have underscored the limitations of these measurements 7

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Flow cytometry of human and mouse single-cell suspensions Cells were stained with surface antibodies and Fixable Viability Dye (Zombie UV, BioLegend) in PBS containing 2% fetal calf serum (FCS) (FACS buffer) for 30 min at 4 C in the presence of human (BioLegend) or mouse (BioXcell) Fc block

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